Vaccinia virus recombinants expressing an 11-kilodalton beta-galactosidase fusion protein incorporate active beta-galactosidase in virus particles
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Tau-beta-galactosidase, an axon-targeted fusion protein.
The most commonly used enzymatic reporter molecule, Escherichia coli beta-galactosidase (beta-gal; beta-D-galactoside galactohydrolase, EC 3.2.1.23), fails to readily diffuse into axons; consequently, the morphologies of beta-gal-labeled neurons cannot directly be determined. For analysis of neuronal pathfinding and synaptic connectivity, this information is essential. We have constructed an ax...
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The beta-galactosidase gene of Streptococcus pneumoniae, bgaA, encodes a putative 2,235-amino-acid protein with the two amino acid motifs characteristic of the glycosyl hydrolase family of proteins. In addition, an N-terminal signal sequence and a C-terminal LPXTG motif typical of surface-associated proteins of gram-positive bacteria are present. Trypsin treatment of cells resulted in solubiliz...
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Gap junctions contain membrane channels that mediate the cell-to-cell movement of ions, metabolites and cell signaling molecules. As gap junctions are comprised of a hexameric array of connexin polypeptides, the expression of a mutant connexin polypeptide may exert a dominant negative effect on gap junctional communication. To examine this possibility, we constructed a connexin 43 (Cx43)/beta-g...
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1.Ausubel, F.M., R. Brent, R.E. Kinston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhl. 1987. Current Protocols in Molecular Biology. Greene/Wiley Interscience, New York. 2.Chandler, C.S. and F.J. Ballard. 1986. Multiple biotin-containing proteins in 3T3-L1 cells. Biochem. J. 237:123-130. 3.Dignam, J., R. Lebovitz and R. Roeder. 1983. Accurate transcription initiation by RNA polymerase II ...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1988
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.62.10.3855-3861.1988